Neurocentre Magendie - Equipe Frick

	Physiopathology of addiction Pier Vincenzo Piazza
	Neurogenesis and pathophysiology Nora Abrous
	Glia-neuron interactions Stéphane Oliet
	Motor system diseases Gwendal Le Masson
	Planar polarity and plasticity Mireille Montcouquiol & Nathalie Sans
	Endocannabinoids and Neuroadaptation Giovanni Marsicano
	Energy Balance and Obesity Daniela Cota
	Cortical Plasticity Andreas Frick
	Pathophysiology of declarative memory Aline Marighetto
	Neuronal circuits of associative learning Cyril Herry


	Guillaume BONY (Phone 3712) PhD, Italian Institute of Technology, Genoa (2011) Postdoc, Neurocentre Magendie (2012)

Keywords:	Corctical Circuitry; Patch-clamp in vivo; Fragile X Syndrome; Sensorial systems; Cell biology;

Skills:	Electrophysiologie: in vitro acute slices, in vivo intracellular patch, patch-clamp recording, préparation solutions Histologie: DAB, biocytin staining, coupe flottante, cryostat, fixation, immunofluorescence, immunohistochimie, microtome à congélation, perfusion rat, perfusion souris, vibratome Microscopie: confocale, image J, neurolucida, photoshop, épifluorescence



We are investigating changes in both the physiological properties and the anatomical connectivity of neurons within neocortical circuits in a mouse model for Fragile X Syndrome. We are using a combination of patch-clamp recording in vivo, single cell transfection and mono-trans-synaptic labelling.



Example of in vivo transfected layer 2/3 pyramidal neuron in WT mouse.